| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pcDNA4/V5-His A |
| 质粒类型: | 哺乳动物细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 5092 bp |
| 5' 测序引物及序列: | T7 Fwd : 5'd[TAATACGACTCACTATAGGG]3' |
| 3' 测序引物及序列: | |
| 载体标签: | 6X His, V5 |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Zeocin (博莱霉素) |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | 用于表达C末端带有V5标签和6xHis标签的蛋白。该载体还易于克隆到其他载体当中去。 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pcDNA4/V5-His A 5092 bp ds-DNA circular SYN 24-OCT-2017
DEFINITION Mammalian vector for expressing C-terminally V5- and 6xHis-tagged
proteins. For other reading frames, use pcDNA(TM)4/V5-His B or
pcDNA(TM)4/V5-His C.
ACCESSION .
VERSION .
KEYWORDS pcDNA4 V5-His A (1)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5092)
AUTHORS Thermo Fisher (Invitrogen)
TITLE Direct Submission
JOURNAL Exported Saturday, November 16, 2019 from SnapGene Viewer 3.1.4
http://www.snapgene.com
COMMENT The cloned gene must include a Kozak sequence and start codon.
FEATURES Location/Qualifiers
source 1..5092
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 235..614
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 895..1008
/note="MCS"
/note="multiple cloning site"
CDS 1009..1050
/codon_start=1
/product="epitope tag from simian virus 5"
/note="V5 tag"
/translation="GKPIPNPLLGLDST"
CDS 1060..1077
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
polyA_signal 1106..1330
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 1376..1804
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1818..2147
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 1998..2133
/note="SV40 ori"
/note="SV40 origin of replication"
promoter 2195..2242
/note="EM7 promoter"
/note="synthetic bacterial promoter "
CDS 2261..2635
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/note="BleoR"
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 2765..2886
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(3337..3925)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4096..4956)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4957..5061)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
961 agatatccag cacagtggcg gccgctcgag tctagagggc ccttcgaagg taagcctatc
1021 cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga
1081 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
1141 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
1201 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
1261 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
1321 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
1381 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
1441 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
1501 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
1561 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
1621 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
1681 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
1741 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
1801 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
1861 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
1921 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
1981 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
2041 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
2101 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
2161 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
2221 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
2281 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
2341 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
2401 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
2461 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
2521 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
2581 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
2641 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
2701 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
2761 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
2821 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
2881 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
2941 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
3001 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
3061 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
3121 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
3181 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
3241 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
3301 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
3361 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
3421 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
3481 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
3541 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
3601 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
3661 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
3721 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
3781 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
3841 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
3901 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
3961 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
4021 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
4081 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
4141 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
4201 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
4261 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
4321 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
4381 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
4441 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
4501 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
4561 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
4621 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
4681 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
4741 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
4801 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
4861 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
4921 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
4981 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
5041 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
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